epicentre Cap Analog Trimethylated C06005 5 U

　　產品名稱:Cap Analog Trimethylated貨號:C06005規格:5 U存儲溫度:-20℃

　　epicentre Cap Analog Trimethylated C06005 5 U產品描述Most naturally occurring eukaryotic mRNAs and many viral RNAs have a modified nucleotide structure known as a“cap”on their 5′terminus. In vivo, the cap structure is involved in the initiation of protein synthesis and in mRNA processing and stability.1 Many viral RNAs are infectious only when capped, and uncapped RNAs introduced into cells via transfection or microinjection are rapidly degraded by cellular RNases.2,3 Therefore,RNA repared for microinjection intooocytes or for transfection into eukaryotic cells should be capped. In vitro, capped mRNAs are translated more efficiently than uncapped transcripts in rabbit reticulocyte lysate or wheat germ translation systems.4 The trimethylated cap is involved in RNA transport5,6 and RNA translation in nematodes.7 Transcripts synthesized for use in in vitro RNA splicing experiments may also need to be capped.8 RNA Cap Analogs Cat. Nos. C32010, C61025, C06005, and C50110 Anti-reverse cap analogs (ARCAs)*, such as m2 7,3′–OG[5′]ppp[5′]G, were found to result in synthesis of transcripts that were more efficiently translated in vitro since the 3′-O-methyl group only permitted them to be incorporated in the correct orientation9,10,11 (see Figure 1). Capping does reduce RNA yields relative to transcription reactions without diguanosine triphosphate, but with the right conditions and high-quality reagents, a large proportion of the transcripts that are made will be capped. For optimum yields of monomethylated, capped in vitro transcripts, we recommend using the AmpliCap-Max?T7 and T3 High Yield Message Maker Kits. EPICENTRE also offers the MessageMAX?T7 ARCA-Capped Message Transcription Kit which is optimized to produce the highest yield of RNA having an ARCA on the 5′end. These kits contain a convenient Cap/NTP PreMix and yield up to 60μg of RNA perμg of Control Template in a standard 30-minute reaction. Capping efficiencies are up to 80%. EPICENTRE’s RNA Cap Analog preparations can also be used with the AmpliScribe?T7, T3, and SP6 High Yield Transcription Kits. Capping reactions with these kits typically yield about 20-40μg of total RNA with ~80% capping efficiency. Protocols for the synthesis ofcapped RNA using AmpliScribe or conventional transcription reagents are given on page 3.

　　epicentre Cap Analog Trimethylated C06005 5 U產品描述大多數天然存在的真核mRNA和許多病毒RNA在其5'末端具有修飾的核苷酸結構，稱為“帽”。在體內，帽結構參與蛋白質合成的啟動以及mRNA的加工和穩定性。1許多病毒RNA僅在加帽后才具有感染力，而通過轉染或顯微注射引入細胞的未蓋帽的RNA會被細胞RNase快速降解。2，3因此，用于顯微注射入卵母細胞或轉染到真核細胞中的RNA應當加蓋。在體外，在兔網織紅細胞裂解液或小麥胚芽翻譯系統中，加帽的mRNA比未加帽的轉錄本更有效地翻譯。4三甲基化帽參與線蟲中RNA的轉運5、6和RNA的翻譯。7合成用于體外RNA剪接實驗的轉錄本也可能需要加帽。8RNA Cap類似物Cat。發現編號為C32010，C61025，C06005和C50110的反反向帽類似物(ARCAs)*，例如m2 7,3'-OG [5'] ppp [5'] G，可以合成由于3'-O-甲基僅允許它們以正確的方向摻入9、10、11(請參見圖1)，因此在體外翻譯效率更高。相對于沒有三磷酸雙鳥苷的轉錄反應而言，加帽的確降低了RNA的產量，但是在適當的條件和高質量的試劑的條件下，大部分制成的轉錄本都將被加帽。為了獲得單甲基化，封端的體外轉錄本的最佳產率，我們建議使用AmpliCap-Max?T7和T3高產率消息產生試劑盒。EPICENTER還提供MessageMAX?T7 ARCA封頂的消息轉錄試劑盒，該試劑盒經過優化，可產生最高產量的5'末端帶有ARCA的RNA。這些試劑盒包含方便的Cap / NTP PreMix，在標準的30分鐘反應中，每微克對照模板可產生高達60微克RNA。封蓋效率高達80%。EPICENTRE的RNA帽類似物制劑也可與AmpliScribe?T7，T3和SP6高產量轉錄試劑盒一起使用。這些試劑盒的加帽反應通常可產生約20-40μg的總RNA，加帽效率約為80%。合成協議封蓋效率高達80%。EPICENTRE的RNA帽類似物制劑也可與AmpliScribe?T7，T3和SP6高產量轉錄試劑盒一起使用。這些試劑盒的加帽反應通常可產生約20-40μg的總RNA，加帽效率約為80%。合成協議封蓋效率高達80%。EPICENTRE的RNA帽類似物制劑也可與AmpliScribe?T7，T3和SP6高產量轉錄試劑盒一起使用。這些試劑盒的加帽反應通常可產生約20-40μg的總RNA，加帽效率約為80%。合成協議第3頁提供了使用AmpliScribe或常規轉錄試劑封端的RNA。

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