檢測認證人脈交流通訊錄
Chroma 激光專用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片
- 主要規格：
- FISH濾光片 TIRF濾光片 Raman濾光片
- 用 途：
- 用于顯微鏡，觀察FISH TIRF Raman現象
- Chroma激光專用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片是常見的集中濾光片類型，屬于對光學品質要求較高的濾光片。
激光專用濾光片用于激光作為光源的顯微鏡，例如激光掃描共聚焦熒光顯微鏡（LSCM），全內反射濾光片（TIRF）用于全內反射熒光顯微鏡（TIRFM），拉曼濾光片用于拉曼成像（Raman），全內反射熒光片用于熒光原位雜交分析（FISH）。
一、熒光顯微鏡 LSCM顯微鏡 TIRFM顯微鏡 FISH熒光原為雜交法 Raman光譜分析
熒光顯微鏡(Fluorescence microscope) ： 熒光顯微鏡是以紫外線為光源， 用以照射被檢物體， 使之發出熒光， 然后在顯微鏡下觀察物體的形狀及其所在位置。熒光顯微鏡用于研究細胞內物質的吸收、運輸、化學物質的分布及定位等。 細胞中有些物質，如葉綠素等，受紫外線照射后可發熒光；另有一些物質本身雖不能發熒光，但如果用熒光染料或熒光抗體染色后，經紫外線照射亦可發熒光，熒光顯微鏡就是對這類物質進行定性和定量研究的工具之一。
激光掃描共聚焦熒光顯微鏡(laser scanning confocal microscopy,LSCM)
測針孔和光源針孔始終聚焦于同一點，使聚焦平面以外被激發的熒光不能進入檢測針孔。
激光共聚焦的工作原理簡單表達就是它采用激光為光源，在傳統熒光顯微鏡成像的基礎上，附加了激光掃描裝置和共軛聚焦裝置，通過計算機控制來進行數字化圖像采集和處理的系統。
TIRFM 全內反射熒光顯微鏡
全內角反射熒光顯微鏡（total internal reflection fluorescence microscope，TIRFM），利用光線全反射后在介質另一面產生衰逝波的特性，激發熒光分子以觀察熒光標定樣品的極薄區域，觀測的動態范圍通常在200 nm以下。因為激發光呈指數衰減的特性，只有極靠近全反射面的樣本區域會產生熒光反射，大大降低了背景光噪聲干擾觀測標的，能幫助研究者獲得高質量的成像質量和可靠的觀測數據。故此項技術廣泛應用于細胞表面物質的動態觀察。
二、激光專用濾光片 FISH濾光片 FITR濾光片 Raman濾光片 NF濾光片的定義
ND濾光片
中性灰度鏡（neutral density filter）又叫中灰密度鏡，簡稱ND鏡，其作用是過濾光線。這種濾光作用是非選擇性的，也就是說，ND鏡對各種不同波長的光線的減少能力是同等的、均勻的，只起到減 弱光線的作用，而對原物體的顏色不會產生任何影響，因此可以真實再現景物的反差。
FISH濾光片
熒光原位雜交技術（Fluorescence in situ hybridization, FISH）是根據已知微生物不同分類級別上種群特異的DNA序列，以利用熒光標記的特異寡聚核苷酸片段作為探針，與環境基因組中DNA分子雜交，檢測該特異微生物種群的存在與豐度。
NF濾光片
陷波濾光片，通常也被稱作帶阻或者帶抑制濾光片。它可以透過絕大多數波長，但是將特定波長范圍內（阻帶）的光衰減到非常低的水平。拉曼光譜用。
Chroma激光專用濾光片 FISH濾光片 FITR濾光片 Raman濾光片的型號參數
熒光濾光片型號 濾光片類型 濾光片說明 注釋（燃料，長通或短通）
Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring
Ha/8-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring
Red-27M AS 27mm diameter, mounted in 1.25" threaded ring
Green-27M AS 27mm diameter, mounted in 1.25" threaded ring
Ha/8-31 AS 31mm diameter, unmounted
Red-31 AS 31mm diameter, unmounted
Blue-31 AS 31mm diameter, unmounted
Green-31 AS 31mm diameter, unmounted
Lum-31 AS 31mm diameter, unmounted
Lum-36 AS 36mm diameter, unmounted
Green-36 AS 36mm diameter, unmounted
Blue-36 AS 36mm diameter, unmounted
Red-36 AS 36mm diameter, unmounted
Ha/8-36 AS 36mm diameter, unmounted
Red-50 AS 50mm diameter, unmounted
Lum-50 AS 50mm diameter, unmounted
Ha/8-50 AS 50mm diameter, unmounted
Green-50 AS 50mm diameter, unmounted
Green-50S AS 50mm x 50mm, square
Blue-50S AS 50mm x 50mm, square
Ha/8-50S AS 50mm x 50mm, square
Lum-50S AS 50mm x 50mm, square
Red-50S AS 50mm x 50mm, square
Ha/8-65S AS 65mm x 65mm, square
Red-65S AS 65mm x 65mm, square
Lum-65S AS 65mm x 65mm, square
Blue-65S AS 65mm x 65mm, square
Green-65S AS 65mm x 65mm, square
Green-27M AS 27mm diameter, mounted in 1.25" threaded ring
Red-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring
Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-31 AS 31mm diameter, unmounted
Green-31 AS 31mm diameter, unmounted
Blue-31 AS 31mm diameter, unmounted
Red-31 AS 31mm diameter, unmounted
Red-36 AS 36mm diameter, unmounted
Blue-36 AS 36mm diameter, unmounted
Lum-36 AS 36mm diameter, unmounted
Green-36 AS 36mm diameter, unmounted
Green-50 AS 50mm diameter, unmounted
Lum-50 AS 50mm diameter, unmounted
Red-50 AS 50mm diameter, unmounted
Red-50S AS 50mm x 50mm, square
Lum-50S AS 50mm x 50mm, square
Green-50S AS 50mm x 50mm, square
Blue-50S AS 50mm x 50mm, square
Green-65S AS 65mm x 65mm, square
Blue-65S AS 65mm x 65mm, square
Red-65S AS 65mm x 65mm, square
Lum-65S AS 65mm x 65mm, square
HQ493/16x EX Spectrum Green?
HQ527/30m EM Spectrum Green?
Q507lp BS Spectrum Green?
HQ548/20x EX Spectrum Orange?
HQ580/14m EM Spectrum Orange?
Q565lp-AR BS Spectrum Orange?
400dclp-AR BS DAPI
D470/40m-AR EM DAPI
AT350/50x EX DAPI
HQ522/40m EM FITC
Q497lp BS FITC
HQ471/39x EX FITC
Q557lp BS Cy3? v1
HQ567/15m EM Cy3? v1
HQ546/11x EX Cy3? v1
HQ565/18m EM Cy3? v2
HQ530/30x EX Cy3? v2
Q553lp BS Cy3? v2
Q593lp BS Cy3.5? v1
HQ581/10x EX Cy3.5? v1
HQ617/40m EM Cy3.5? v1
HQ617/40m EM Cy3.5? v2
HQ580/13x EX Cy3.5? v2
Q593lp BS Cy3.5? v2
Q647lp BS Cy5? v1
HQ622/36x EX Cy5? v1
HQ667/30m EM Cy5? v1
HQ667/30m EM Cy5? v2
HQ630/20x EX Cy5? v2
Q649lp BS Cy5? v2
Q697lp BS Cy5.5?
HQ682/12x EX Cy5.5?
HQ721/42m EM Cy5.5?
HQ740/35x EX Cy7?
HQ795/50m EM Cy7?
Q765lp BS Cy7?
Q615lp BS Texas Red?
HQ631/23m EM Texas Red?
HQ602/13x EX Texas Red?
HQ782/20x EX Laser-Pro
HQ817/25m EM Laser-Pro
Q800lp BS Laser-Pro
ET460/50m EM ET - 405nm Laser Bandpass Set for TIRF applications
ZET405/20x EX ET - 405nm Laser Bandpass Set for TIRF applications
ZT405rdc BS ET - 405nm Laser Bandpass Set for TIRF applications
ET425lp EM ET - 405nm Laser Bandpass Set for TIRF applications
ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications
ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications
ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications
ET480/40m EM ET - 440nm Laser Bandpass Set for TIRF applications
ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications
ET500/40m EM ET - 440nm Laser Bandpass Set for TIRF applications
ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications
ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications
ZT488rdc BS ET - 488nm Laser Bandpass Set for TIRF applications
ET500lp EM ET - 488nm Laser Bandpass Set for TIRF applications
ET525/50m EM ET - 488nm Laser Bandpass Set for TIRF applications
ZET488/10x EX ET - 488nm Laser Bandpass Set for TIRF applications
ZT514rdc BS ET - 514nm Laser Bandpass Set for TIRF applications
ZET514/10x EX ET - 514nm Laser Bandpass Set for TIRF applications
ET545/40m EM ET - 514nm Laser Bandpass Set for TIRF applications
ET525lp EM ET - 514nm Laser Bandpass Set for TIRF applications
ET542lp EM ET - 532nm Laser Bandpass Set for TIRF applications
ET575/50m EM ET - 532nm Laser Bandpass Set for TIRF applications
ZT532rdc BS ET - 532nm Laser Bandpass Set for TIRF applications
ZET532/10x EX ET - 532nm Laser Bandpass Set for TIRF applications
ZET532/10x EX ET - 532nm Laser Bandpass Set for TIRF applications
ZT532rdc BS ET - 532nm Laser Bandpass Set for TIRF applications
ET590/50m EM ET - 532nm Laser Bandpass Set for TIRF applications
ET542lp EM ET - 532nm Laser Bandpass Set for TIRF applications
ZET561/10x EX ET - 561nm Laser Bandpass Set for TIRF applications
ZT561rdc BS ET - 561nm Laser Bandpass Set for TIRF applications
ET600/50m EM ET - 561nm Laser Bandpass Set for TIRF applications
ET575lp EM ET - 561nm Laser Bandpass Set for TIRF applications
ET575lp EM ET - 561nm Laser Bandpass Set for TIRF applications
ZT561rdc BS ET - 561nm Laser Bandpass Set for TIRF applications
ZET561/10x EX ET - 561nm Laser Bandpass Set for TIRF applications
ET630/75m EM ET - 561nm Laser Bandpass Set for TIRF applications
ZT594rdc BS ET - 594nm Laser Bandpass Set for TIRF applications
ET645/75m EM ET - 594nm Laser Bandpass Set for TIRF applications
ZET594/10x EX ET - 594nm Laser Bandpass Set for TIRF applications
ET610lp EM ET - 594nm Laser Bandpass Set for TIRF applications
ET655lp-TRF EM ET - 633-640nm Laser Longpass Set for TIRF applications
ZET635/20x EX ET - 633-640nm Laser Longpass Set for TIRF applications
ZT640rdc BS ET - 633-640nm Laser Longpass Set for TIRF applications
ZET642/20x EX ET - 640-647nm Laser Longpass Set for TIRF applications
ZT647rdc BS ET - 640-647nm Laser Longpass Set for TIRF applications
ET665lp EM ET - 640-647nm Laser Longpass Set for TIRF applications
ET705/72m EM ET - 640-647nm Laser Longpass Set for TIRF applications
ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488m-TRF EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications
ET450/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET442/514m-TRF EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ZT442/514rpc BS ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514x EX ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514x EX ET – 442/514nm Laser Dual Band Set for TIRF applications
ZT442/514rpc BS ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ET545/40m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ET480/40m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/532x EX ET – 442/532nm Laser Dual Band Set for TIRF applications
ZT442/532rpc BS ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532m-TRF EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ET585/65m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ZT442/532rpc BS ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532x EX ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ET500/40m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET488/561x EX ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561m-TRF EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZT488/561rpc BS ET – 488/561nm Laser Dual Band Set for TIRF applications
ZT488/561rpc BS ET – 488/561nm Laser Dual Band Set for TIRF applications
ET610/75m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561x EX ET – 488/561nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/594x EX ET – 488/594nm Laser Dual Band Set for TIRF applications
ZT488/594rpc BS ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594m-TRF EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZT488/594rpc BS ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594x EX ET – 488/594nm Laser Dual Band Set for TIRF applications
ET645/75m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/640x EX ET – 488/640nm Laser Dual Band Set for TIRF applications
ZT488/640rpc BS ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640m-TRF EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZT488/640rpc BS ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640m EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640x EX ET – 488/640nm Laser Dual Band Set for TIRF applications
ET655lp EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET532/640x EX ET – 532/640nm Laser Dual Band Set for TIRF applications
ZT532/640rpc BS ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640m-TRF EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ET585/65m EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ZT532/640rpc BS ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640x EX ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640m EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ET655lp EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET405/488/561x EX ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZT405/488/561rpc BS ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ET575lp EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZET488/561m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ET525/50m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZT405/488/561rpc BS ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561x EX ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561m-TRF EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561x EX ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZT405/488/561rpc BS ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET575lp EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET450/50m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET525/50m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/594x EX ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZT405/488/594rpc BS ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594m-TRF EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZT405/488/594rpc BS ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594x EX ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET535/70m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET450/50m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET645/75m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET442/514/561x EX ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT442/514/561rpc BS ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561m-TRF EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET540/30m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT442/514/561rpc BS ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561x EX ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET575lp EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET480/40m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT405/488/561/640rpc BS ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640x EX ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640m-TRF EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET450/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET525/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZT405/488/561/640rpc BS ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640x EX ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET655lp EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET595/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZT405/488/561/647rpc BS ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647x EX ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647m-TRF EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET700/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647x EX ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZT405/488/561/647rpc BS ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET450/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET525/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET605/52m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
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