檢測認證人脈交流通訊錄
- 正常人主要細胞溶解產物 Preparation：Cell lysate was prepared in modified RIPA buffer (50mMTris, pH7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40 , 1mM Na3VO4, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted and boiled for 5 min with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol. Concentration：2 mg/ml Storage: Stable for 6 months at -20oC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing. Application： Cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Cat.No. Product Name Size Price 1106 Human brain vascular smooth muscle cell lysate 100 µg $330 人腦血管平滑肌細胞 1206 Human brain vascular pericyte lysate 100 µg $330 人腦血管周邊細胞 1406 Human meningeal cell lysate 100 µg $330 人腦膜細胞 1526 Human neuron lysate 100 µg $330 人神經(jīng)元細胞 1606 Human oligodendrocyte precursor cell lysate 100 µg $330 人少突先驅膠質細胞 1716 Human perineurial cell lysate 100 µg $330 人周神經(jīng)細胞 1806 Human astrocyte lysate 100 µg $330 人星形膠質細胞 1906 Human microglia lysate 100 µg $330 人小膠質細胞 2006 Human dermal microvascular endothelial cell lysate 100 µg $330 人皮膚微血管內皮細胞 2016 Human dermal vascular smooth muscle cell lysate 100 µg $330 人皮膚血管平滑肌細胞 2106 Human dermal kerotinocyte lysate 100 µg $330 人皮膚角質細胞 2206 Human dermal melanocyte lysate 100 µg $330 人皮膚黑素細胞 2306 Human dermal fibroblast lysate 100 µg $330 人皮膚纖維原細胞 2506 Human lymphatic endothelial cell lysate 100 µg $330 人淋巴內皮細胞 2536 Human lymphatic fibroblast lysate 100 µg $330 人淋巴纖維原細胞 2626 Human gingival fibroblast lysate 100 µg $330 人齒齦纖維原細胞 2636 Human peridontal ligmant firbroblast lysate 100 µg $330 人齒根膜韌帶纖維原細胞 2716 Human esophageal smooth muscle cell lysate 100µg $330 人食管平滑肌細胞 2906 Human intestinal microvascular endothelial cell lysate 100 µg $330 人腸微血管內皮細胞 2916 Human intestinal smooth muscle cell lysate 100 µg $330 人腸平滑肌細胞 3006 Human pulmonary microvascular endothelial cell lysate 100 µg $330 人肺微血管內皮細胞 3106 Human pumonary artery endothelial cell lysate 100 µg $330 人肺動脈內皮細胞 3116 Human pulmonary artery smooth muscle cell lysate 100 µg $330 人肺動脈平滑肌細胞 3126 Human pulmonary artery fibroblast lysate 100 µg $330 人肺動脈纖維原細胞 3206 Human pulmonary alveolar epithelial cell lysate 100 µg $330 人肺齒槽上皮細胞 3306 Human pulmonary fibroblast lysate 100 µg $330 人肺纖維原細胞 3406 Human bronchial smooth muscle cell lysate 100 µg $330 人支氣管平滑肌細胞 3416 Human tracheal smooth muscle cell lysate 100 µg $330 人氣管平滑肌細胞 3506 Human skeletal muscle cell lysate 100 µg $330 人骨骼肌細胞 4006 Human renal glomerular endothelial cell lysate 100 µg $330 人腎小球內皮細胞 4106 Human renal proximal tubular epithelial cell lysate 100 µg $330 人腎最接近管狀上皮細胞 4116 Human renal cortical epithelial cell lysate 100 µg $330 人腎皮層上皮細胞 4126 Human renal epithelial cell lysate 100 µg $330 人腎上皮細胞 4206 Human renal mesangial cell lysate 100 µg $330 人腎系膜細胞 4306 Human bladder microvascular endothelial cell lysate 100 µg $330 人膀胱微血管內皮細胞 4316 Human bladder smooth muscle cell lysate 100 µg $330 人膀胱平滑肌細胞 4336 Human bladder stromal fibroblast lysate 100 µg $330 人膀胱基質纖維原細胞 4606 Human calvarial osteoblast lysate 100 µg $330 人顱蓋造骨細胞 5006 Human hepatic sinusoidal endothelial cell lysate 100 µg $330 人肝竇狀腺內皮細胞 5106 Human intraheptic biliary epithelial cell lysate 100 µg $330 人肝膽上皮細胞 5206 Human hepatocyte lysate 100 µg $330 人肝細胞 6006 Human cardiac microvascular endothelial cell lysate 100 µg $330 人心臟微血管內皮細胞 6106 Human aortic endothelial cell lysate 100 µg $330 人大動脈內皮細胞 6116 Human aortic smooth muscle cell lysate 100 µg $330 人大動脈平滑肌細胞 6206 Human cardiac myocyte lysate 100 µg $330 人心肌細胞 6526 Human keratocyte lysate 100 µg $330 人角膜細胞 6546 Human retinal pigment epithelial cell lysate 100 µg $330 人視網(wǎng)膜色素上皮細胞 6556 Human lens epithelial cell lysate 100 µg $330 人視鏡上皮細胞 6566 Human iris pigment epithelial cell lysate 100 µg $330 人虹膜色素上皮細胞 6586 Human non-pigment ciliary epithelial cell lysate 100 µg $330 人非色素睫毛上皮細胞 7106 Human amniotic epithelial cell lysate 100 µg $330 人羊膜上皮細胞 7126 Human villous trophoblast lysate 100 µg $330 人長茸毛滋養(yǎng)層 7136 Human villous mesenchymal fibroblast lysate 100 µg $330 人長茸毛間葉纖維原細胞 7216 Human preadipocyte lysate 100 µg $330 人前脂肪細胞 8006 Human umbilical vein endothelial cell lysate 100 µg $330 人臍靜脈內皮細胞 8016 Human umbilical artery endothelial cell lysate 100 µg $330 人臍動脈內皮細胞 8026 Human umbilical vein smooth muscle cell lysate 100 µg $330 人臍靜脈平滑肌細胞 8036 Human umbilical artery smooth muscle cell lysate 100 µg $330 人臍動脈平滑肌細胞
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