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Cell lysate run on 12% SDS-PAGE and transferred to PVDF.? Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable).

細(xì)胞裂解物在12% SDS-PAGE電泳并轉(zhuǎn)膜（PVDF）。一抗室溫孵育1小時(shí)（過(guò)夜孵育也可）。

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REFERENCES:

參考文獻(xiàn)：

Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.

Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.

Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation.? PLoS Pathog 2012; 8 (4).

Grisé H, Frausto S, Logan T, Tang H. A conserved tandem cyclophilin-binding site in hepatitis C virus nonstructural protein 5A regulates Alisporivir susceptibility. J Virol. 2012 May;86(9):4811-22

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